What is uncut lambda DNA?
Lambda DNA, a linear, double-stranded phage DNA containing 12 bp single-stranded complementary 5′-ends, is derived from an Escherichia coli bacteriophage (Bacteriophage lambda cI857 Sam7). Lambda DNA can also be used as a substrate in restriction enzyme activity assays. …
How many base pairs long is lambda?
49130 base pairs
Phage lambda DNA is a double-stranded, linear molecule, 49130 base pairs in length.
How many nucleotides does bacteriophage lambda?
A DNA sequence for the genome of bacteriophage phi X174 of approximately 5,375 nucleotides has been determined using the rapid and simple ‘plus and minus’ method.
Is lambda DNA circular or linear?
Our lambda DNA is linear. We do not sell Lambda DNA in a circular form. Theoretically, it could be ligated to form circles but, due to its large size, it is difficult to achieve.
How many ecor1 sites are there in lambda DNA?
5 recognition sites
The Lambda DNA used in this experiment is isolated as a linear molecule from the E. coli bacteriophage lambda. It contains approximately 49,000 base pairs and has 5 recognition sites for Eco RI, and 7 for Hind III.
Why does lambda DNA contain multiple bands?
After electrophoresis, the gel band pattern of a Lambda DNA digest may show an extra band, due to the joining (and forming the cos-site) of the two end fragments. When the digest is not heated before the gel run (lane 1), a number of b and c fragments have joined to form band a, which is in size the sum of b and c.
How many base pairs does bacteriophage lambda?
48,502 base pairs
In its circular form, the phage genome, therefore, is 48,502 base pairs in length. The lambda genome can be inserted into the E. coli chromosome and is then called a prophage. See section below for details.
How many nucleotides present in PI 174 and phage lambda respectively?
5386 nucleotides →7ϕ×174 bacteriophage p.
How many ecor1 palindromic sequences are found in the λ phage’s genome?
coli bacteriophage lambda. It contains approximately 49,000 base pairs and has 5 recognition sites for Eco RI, and 7 for Hind III. The smaller fragments generated by a restriction enzyme, such as those generated by Hind III, may not be visible after agarose gel electrophoresis.
What is the length of Cos site?
cos. The cos site is a ≈200 bp long segment that is required to both initiate and terminate the packaging of a monomeric genome from concatemeric DNA. The site where terminase introduces staggered nicks to generate the cohesive ends is called cosN (fig. 2).
Is lambda DNA double stranded?
The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded complementary 5′-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining.
Who determine the sequence of lambda phage?
Explanation: The phage DNA molecule, unlike the bacterial plasmid DNA, exists usually in a linear conformation and DNA is a duplex molecule. 3. Who determined the sequence of Lambda phage? Explanation: The exact sequencing of lambda phage of 49 kb was done with Sanger sequencing method in 1982.