What is an expression system?
Definition. Expression systems are genetic constructs (a gene encoded by DNA) that are designed to produce a protein, or an RNA (ribonucleic acid), either inside or outside a cell. Expression systems are used in research and in the commercial production of enzymes or therapeutics.
What is the difference between promoter and operator?
Promoter vs Operator Promoters are the sites in which RNA polymerase binds and they are present upstream of the transcription start site of a gene. Operators are the sites in which the regulatory molecule binds into an operon model.
What are bacterial expression systems?
A bacterial host is the most widely used expression system due to its simple procedure and low cost. One-stop shopping from gene synthesis or gene cloning, expression, and purification. Optimized expression with inducer, temperature, and time course.
What are available expression systems?
The various protein expression systems are bacteria, yeast, insect or mammalian systems.
What is Bicistronic lentiviral vector?
Lenti-X bicistronic lentiviral vectors and expression systems allow you to deliver and stably express your gene of interest, along with a coexpressed bright fluorescent protein or an antibiotic selection marker, in virtually any mammalian cell type.
What is ef1a promoter?
The EF‐1α promoter is known as one of the strongest promoters in various mammalian cell lines 32, and the CAG promoter has been used frequently to drive strong gene expression in mammalian cells.
How does the Lenti X bicistronic expression system work?
The Lenti-X Bicistronic Expression Systems enable you to produce high-titer recombinant lentivirus for the purpose of transferring genes into dividing or non-dividing mammalian cells.
When do you use a bicistronic expression vector?
Martinez-Salas E. Internal ribosome entry site biology and its use in expression vectors. Curr Opin Biotechnol. 1999 Oct;10 (5):458-64. D Trouet, B Nilius, T Voets, G Droogmans, J Eggermont. Use of a bicistronic GFP-expression vector to characterise ion channels after transfection in mammalian cells.
When do you need a bicistronic or IRES element?
Bicistronic or Internal Ribosome Entry Site (IRES) Element — Efficient co-expression of two genes in a single vector. The IRES element is exactly what’s needed for certain experiments when a single-gene vector falls short. IRES enables the coordinated co-expression of two genes with the same vector.
Which is a key element of a bicistronic lentiviral vector?
The key element of these bicistronic lentiviral vectors is an optimized internal ribosome entry site (IRES) that permits your gene of interest and a second detectable protein to be coexpressed from a single mRNA transcript.