What is goat anti-Mouse IgG?
Goat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB. ELISA and Immunohistochemistry: A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry. Recognizes mouse IgG, both heavy and light chains.
What is the role of goat anti rabbit IgG?
Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.
What does goat anti-mouse antibody mean?
Anti-mouse secondary antibodies are generated by immunizing a host animal (e.g., a goat) with a pooled population of immunoglobulins (Ig) from the target species (i.e., mice). These antibodies are designated as cross-adsorbed or highly cross-adsorbed, based on the amount of processing which has occurred.
What is IgG control?
Summary and Explanation: Negative Control Mouse IgG is used in place of a primary mouse monoclonal antibody with a section of each patient specimen to evaluate nonspecific staining. The Negative Control Mouse IgG is intended for use as a negative control with mouse monoclonal antibodies.
What is irrelevant IgG?
The term “irrelevant” refers to a primary antibody of the same isotype as the specific primary antibody (i.e. mouse IgG) and applied at the same concentration, that is known not to bind to a target in the tissue specimen.
What is the role of goat anti-rabbit IgG HRP conjugate in Elisa?
Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA. Highly purified whole rabbit IgG. Specific for rabbit IgG heavy and light chains. Evaluated by western blot.
What are the features of Goat anti mouse antibody?
Key features and details 1 Goat Anti-Mouse IgG H&L 2 Host species: Goat 3 Isotype: IgG 4 Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB
How long to incubate Goat anti mouse antibody ab6708?
Both heavy (55kDa) and light (28kDa) chains are detected. Lysates/proteins at 0.05 µg per lane. ab6708 incubated for 1 hour at room temperature. To our knowledge, customised protocols are not required for this product.
What should I do with my goat IgG antibody?
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Antiserum to mouse IgG (H/L) was raised by repeated immunisation of goats with highly purified antigen.
Are there any secondary antibodies for Chicken IgG?
We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.