How does Seahorse XF analyzers work?
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) at intervals of approximately 5-8 minutes. The instrument measures the concentrations for 2-5 minutes then calculates the OCR and ECAR, respectively.
How much does a seahorse analyzer cost?
$742.50 / month Click to Apply Now!
What does Seahorse measure?
Seahorse XF technology measures the flux of oxygen, the oxygen consumption rate [OCR], and the flux of protons, the extracellular acidification rate [ECAR], in the medium immediately surrounding cells in a microplate. OCR is proportional to mitochondrial respiration, while ECAR is proportional to glycolysis.
How does Seahorse measure oxygen?
Seahorse XF extracellular flux analyzer is an alternative to the Clark type electrode. It was first introduced in 2006. By using the optical sensors, it simultaneously measures the proton and oxygen level in a very small volume of media above a monolayer of cultured cells.
What is the seahorse assay?
The Agilent Seahorse XF ATP Real-Time rate assay measures and quantifies the rate of ATP production from glycolytic and mitochondrial system simultaneously using label-free technology in live cells. Seahorse XF technology measures the flux of both H+ production (ECAR) and O2 consumption (OCR), simultaneously.
How do you calculate spare respiratory capacity?
Spare respiratory capacity (Maximal OCR – Basal OCR) is the ability of an organism to respond to increasing energy demands. To calculate, subtract each well’s average basal OCR from its corresponding average FCCP response (maximal OCR).
What is extracellular acidification rate?
Extracellular acidification is an easily measured indication of cellular metabolic rate. In cells where the pentose phosphate pathway is highly active, the use of pathway inhibitors such as 6-aminonicotinamide may be useful to isolate glycolytic rate.
What is glycolytic capacity?
Glycolytic capacity is a measure of the maximum rate of conversion of glucose to pyruvate or lactate that can be achieved acutely by a cell. Since glycolytic ATP synthesis is obligatorily linked to glycolytic carbon flux, glycolytic capacity is also a measure of the maximum capacity of glycolysis to generate ATP.
What is mitochondrial OCR?
Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function.
What is Mito Stress Test?
The Seahorse XF Cell Mito Stress Test is the gold standard assay and is widely used for measuring mitochondrial function in cells. This assay provides insight into the cause of mitochondrial dysfunction and an in-depth understanding of metabolic pathways, signals, and phenotypes.
How do you measure OCR?
Oxygen consumption rate (OCR) is measured before and after the addition of inhibitors to derive several parameters of mitochondrial respiration. Initially, baseline cellular OCR is measured, from which basal respiration can be derived by subtracting non-mitochondrial respiration.
What does a XRF analyzer do?
XRF (X-ray fluorescence) is a non-destructive analytical technique used to determine the elemental composition of materials. XRF analyzers determine the chemistry of a sample by measuring the fluorescent (or secondary) X-ray emitted from a sample when it is excited by a primary X-ray source.
What can a XRF analyze?
An X-ray fluorescence (XRF) spectrometer is an x-ray instrument used for routine, relatively non-destructive chemical analyses of rocks, minerals, sediments and fluids. It works on wavelength-dispersive spectroscopic principles that are similar to an electron microprobe (EPMA).
What is XRF and how does XRF work?
XRF is an acronym for X-ray fluorescence spectroscopy. XRF is a non-destructive analytical technique used to determine the elemental composition of materials. Handheld XRF analyzers work by measuring the fluorescent (or secondary) X-rays emitted from a sample when excited by a primary X-ray source.
How to XRF works?
Understanding X-Ray Fluorescence: How Does XRF Work? Emission. First, the analyzer emits X-rays. Excitation. The X-rays hit the sample, causing it to fluoresce and send X-rays back to the analyzer. Measurement. The detector measures the energy spectrum. Results. Olympus Axon Technology™ processes the energy spectrum and displays the sample’s elemental composition.