Is BCR-ABL scale?
We aligned BCR-ABL values generated by 38 laboratories to an international scale (IS) where a major molecular response (MMR) is 0.1% or less. Alignment was achieved by application of laboratory-specific conversion factors calculated by comparisons performed with patient samples against a reference method.
What does BCR-ABL measure?
The presence of the gene sequence known as BCR-ABL1 confirms the diagnosis of CML and a form of acute lymphoblastic lymphoma (ALL), specifically a type of B-lymphoblastic leukemia/lymphoma. In very rare cases, the abnormal chromosome is linked to cases of acute myeloid leukemia and T-lymphoblastic leukemia/lymphoma.
What is BCR code?
The BCR gene provides instructions for making a protein whose function is not completely understood. Studies show that the BCR protein may act as a GTPase activating protein (GAP). GAPs turn off (inactivate) proteins called GTPases, which play an important role in chemical signaling within cells.
What is BCR lab test?
A BCR-ABL genetic test looks for a genetic mutation (change) on a specific chromosome. Chromosomes are the parts of your cells that contain your genes. Genes are parts of DNA passed down from your mother and father. They carry information that determines your unique traits, such as height and eye color.
What is BCR-ABL1 test for?
A BCR-ABL test is most often used to diagnose or rule out chronic myeloid leukemia (CML) or a specific form of acute lymphoblastic leukemia (ALL) called Ph-positive ALL. Ph-positive means a Philadelphia chromosome was found. The test is not used to diagnose other types of leukemia.
Which is the best Test to detect BCR-ABL1?
Currently, real-time quantitative PCR (RQ-PCR) is the most commonly used test for assessing MR in patients with CML and is able to detect BCR-ABL1 transcript levels down to MR 4.0 to MR 4.5 on the IS. It has been reported that dPCR can detect BCR-ABL1 with greater sensitivity and precision than RQ-PCR.
What kind of DNA is used in NIBSC-BCR ABL1?
Samples are presented as approximately 1.5x10E6 cells freeze-dried genomic DNA in glass ampoules. The collaborative validation study data indicates that these materials are suitable for use in RNA extraction and subsequent Q-PCR assays to quantitate the BCR-ABL1 fusion gene transcript.
What causes the fusion of BCR and ABL1?
Nearly all cases of CML and a minority of cases of ALL are caused by a t (9;22) (q34;q11) chromosome translocation – known as the Philadelphia chromosome – which fuses 2 genes: BCR and ABL1. The BCR-ABL1 fusion acts as an oncogene and promotes genomic instability.
How are BCR and Gus is alignments validated?
The BCR and GUS IS alignments are currently in process. All 3 assays were validated for linearity, precision, accuracy, limit of detection, and limit of blank, following in vitro diagnostics industry best practices and Clinical and Laboratory Standards Institute (CLSI) guidelines.