What color is Alexa Fluor 488?
green-
Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line.
Why is DyLight 488 Essential?
DyLight® 488 is a green fluorophore commonly used in applications such as protein labeling, immunolabeling, fluorescence microscopy, and enzyme immunoassays. DyLight® 488 is bright and photostable, and can be used as a substitute for other green fluorophores such as FITC.
What is the difference between fluorophore and fluorochrome?
As nouns the difference between fluorochrome and fluorophore is that fluorochrome is any of various fluorescent dyes used to stain biological material before microscopic examination while fluorophore is (biochemistry) a molecule or functional group which is capable of fluorescence.
What is Alexa Fluor Plus?
Invitrogen Alexa Fluor Plus conjugate technology provides higher signal to noise ratios with minimized cross-reactivity for fluorescent imaging and western blotting.
What is the absorption limit of Alexa Fluor 488?
Alexa 488 absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell and tissue staining of multiple proteins with Alexa Fluor ® 594 and Alexa Fluor ® 647 conjugated secondary antibodies.
What is the wavelength range of Alexa Fluor dyes?
The yellow-fluorescent Alexa Fluor 532 dye (excitation/emission maxima ~532/554 nm) absorbs in the wavelength range between the green-fluorescent Alexa Fluor 488 dye and orange-fluorescent Alexa Fluor 546 dye ( Figure 1.3.2) and provides strong visible fluorescence that contrasts well with these dyes.
Can you use Alexa Fluor 488 in flow cytometry?
Alexa Fluor 488 conjugates are compatible with common fluorescein equipment, settings, or filters and are ideally suitable for all applications in fluorescence microscopy and flow cytometry with high requirements in sensitivity.
How are Alexa Fluor 488 conjugated antibodies different from FITC?
Alexa Fluor® 488-conjugated antibodies Alexa Fluor ® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining. Comparable spectral properties and quantum yield. Brighter and more photostable when conjugated to antibodies.