What is principle of HPLC chromatography?
The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Depending on the chemical structure of the analyte, the molecules are retarded while passing the stationary phase.
What is the basis of HPLC?
HPLC works following the basic principle of thin layer chromatography or column chromatography, where it has a stationary phase ( solid like silica gel) and a mobile phase (liquid or gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it.
What are the types of HPLC chromatography?
HPLC analyses fall into four different categories: (1) reverse phase chromatography where the stationary phase is hydrophobic (C18-modified silica), (2) normal phase chromatography where the stationary phase is hydrophilic (silica), (3) hydrophilic interaction chromatography, a hybrid of techniques 1 and 2, and (4) ion …
What is HPLC and its types?
Different types of HPLC columns are used in analysis of different pharmaceutical compounds according to their nature and column separation capacity. Therefore silica columns can be used to analyze the compounds of different chemical natures. The material filled in the HPLC columns is known as a stationary phase.
What is HPLC instrument?
High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size.
Why is HPLC important?
The HPLC is used in the quantitative determination of plasma levels of drugs and their metabolites. This is of special importance during the development of new drugs and for monitoring therapy. The measurement of concentrations of certain biogenic substances helps in diagnosis.
What are the parts of HPLC?
Main High-Performance Liquid Chromatography (HPLC) Components. The HPLC system mainly consists of an infusion pump, a sampler, a chromatographic column, a detector, and a data recording and processing device. Among them, the infusion pump, the chromatographic column, and the detector are key components.
What are the advantages of gas chromatography over HPLC?
Advantages of gas chromatography compared to HPLC. 1, high sensitivity 2, the range of samples that can be detected is relatively wide 3 , the reagents can be a lot of discount, the liquid phase is more chromatographically pure, the gas phase is more analytically pure
What are the advantages of HPLC over GC?
Mobile Carrier Phase. Gas chromatography vaporizes the sample and it is carried along the system by an inert gas such as helium.
What is basic principle of HPLC?
HPLC Theory. HPLC works following the basic principle of thin layer chromatography or column chromatography, where it has a stationary phase ( solid like silica gel) and a mobile phase (liquid or gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it.
What is the HPLC used for?
Pharmaceutical HPLC Equipment HPLC (known as high performance liquid chromatography, high pressure liquid chromatography) is used to separate the phases of a solid or liquid analyte, regardless of its stability and volatility. HPLC equipment is popular for its regarded ease of set up, use, and configuration potential.