What was the purpose of 35S methionine labeling?
Pulse-chase labeling of antigens with [35S]methionine is used to determine the relative half-life of a protein.
What amino acid can be labeled with 35S?
methionine
[35S]methionine is the radiolabeled amino acid of choice for metabolic labeling of proteins because of its high specific activity (>800 Ci/mmol) and ease of detection.
What does S methionine do?
Methionine is a unique sulfur-containing amino acid that can be used to build proteins and produce many molecules in the body. These include the antioxidant glutathione and the molecule SAM, which is used to modify DNA and other molecules.
What is s35 labeling?
Overview. Metabolic labeling of cells with low-energy beta-emitting radioisotopes such as 35S-methionine and 35S-methionine/cysteine is often used to follow the biosynthesis, maturation, and degradation of proteins in vivo.
Is DL methionine safe for humans?
It is POSSIBLY UNSAFE to use methionine by mouth or intravenously to self-medicate. Too much methionine can cause brain damage and death. Methionine can increase blood levels of homocysteine, a chemical that may cause heart disease.
Are 35S stable?
Sulfur-35 (35S), the most stable radioactive isotope of sulfur, used in biochemical research to radioactively label proteins.
What is the RDA for methionine?
The RDA for methionine (combined with cysteine) for adults has been set at 14 mg/Kg of body weight per day. Therefore a person weighing 70 Kg, independent of age or sex, requires the consumption of around 1.1 g (0.9 mMoles) of methionine/cysteine per day.
How is radiolabeling performed?
Radiolabeling is a process that is frequently used in medicine, drug research and development, and environmental case studies. Using this process, researchers can track the movement or breakdown of target molecules. To accomplish this, atoms within the target molecule are replaced by isotopes of the same atom.