What is the reference method for measuring blood glucose?
Glucose is measured by various enzymatic methods. The hexokinase/glucose-6-phosphate dehydrogenase (G6PD) method developed by the American Association for Clinical Chemistry has been accepted as the reference method for glucose determination.
What is the reference value of glucose?
The expected values for normal fasting blood glucose concentration are between 70 mg/dL (3.9 mmol/L) and 100 mg/dL (5.6 mmol/L). When fasting blood glucose is between 100 to 125 mg/dL (5.6 to 6.9 mmol/L) changes in lifestyle and monitoring glycemia are recommended.
What electrochemical method is used in a glucose measurement?
The hexokinase assay is the reference method for measuring glucose using spectrophotometry in many clinical laboratories [32]. Glucose biosensors for SMBG are usually based on the two enzyme families, GOx and GDH. These enzymes differ in redox potentials, cofactors, turnover rate and selectivity for glucose [33].
What is a continuous glucose monitoring device?
Continuous glucose monitoring (CGM) devices help you manage Type 1 or Type 2 diabetes with fewer fingerstick tests. A sensor just under your skin measures your glucose levels 24 hours a day. A transmitter sends results to a wearable device or cell phone.
What is glucose oxidase method?
In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen peroxide. The hydrogen peroxide is then oxidatively coupled with a chromogen to produce a colored compound which may be measured spectroscopically.
What form of glucose is measured by glucose oxidase method?
Although glucose oxidase is specific for β-d-glucose, solutions of d-glucose can be quantified, because α-d-glucose will mutorotate to β-d-glucose as the β-d-glucose is consumed by the enzymatic reaction. Thus, this enzyme is also used in biosensors that measure the amount of glucose in blood.
What is mg dL means?
Milligrams per decilitre (mg/dL) Some medical tests report results in milligrams (mg) per decilitre (dL). A milligram is one-thousandth of a gram. A gram is about 1/30 of an ounce. A decilitre measures fluid volume that is 1/10 litre.
What is RBS test for?
A random blood sugar test is the testing of the blood sugar level at any time or random time of the day. It is a test performed outside the regular testing schedule. RBS test is performed to confirm diabetes mellitus, during the treatment and after the treatment of diabetes mellitus.
What are the 2 categories of methods in glucose determination?
There are three basic approaches to the laboratory measurement of blood glucose concentration: reducing methods, condensation methods, and enzymatic methods. Reducing methods are the oldest and take advantage of the reducing properties of glucose to change the state of a metal ion while glucose is being oxidized.
What is electrochemical glucose sensor?
The glucose sensor is an amperometric electrochemical biosensor generating a current from the electrochemical reaction between glucose and a glucose oxidase layer on working electrode (WE). The use of iridium-oxide nanoparticles helps for the transfer of the electrons from the glucose oxidase to WE.
What is the difference between Flash glucose monitoring and CGM?
CGM monitors your sugar levels continuously and sends data to your display device (a hand held monitor or pump). So you can set alerts for high, low or rate of change. With flash glucose monitoring it’s only when you scan your sensor that you get your reading and trends.
Is FreeStyle Libre 2 a CGM?
The FreeStyle Libre 2 Flash Glucose Monitoring System is a continuous glucose monitoring (CGM) device with real time alarms capability indicated for the management of diabetes in persons age 4 and older.