What does eIF4F do?

What does eIF4F do?

The eIF4F complex comprises the cap binding protein, eIF4E, the helicase eIF4A, and the large scaffolding protein eIF4G and is responsible for recruiting ribosomal subunits to the initiation codon of an mRNA.

What does eIF4F bind to?

messenger RNAs
Eukaryotic initiation factor 4F (eIF4F) is a heterotrimeric protein complex that binds the 5′ cap of messenger RNAs (mRNAs) to promote eukaryotic translation initiation.

Is the Kozak sequence in the 5 UTR?

The prokaryotic 5′ UTR contains a ribosome binding site (RBS), also known as the Shine–Dalgarno sequence (AGGAGGU), which is usually 3–10 base pairs upstream from the initiation codon. In contrast, the eukaryotic 5′ UTR contains the Kozak consensus sequence (ACCAUGG), which contains the initiation codon.

Do I need a Kozak sequence?

Yes it is advisable to include them in (eukaryotic expression system) cDNA. The Kozak consensus sequence plays a major role in the initiation of the translation process. You may get poor amount of protein if the sequence is not included.

What is Kozak rule?

The Kozak consensus sequence (Kozak consensus or Kozak sequence) is a nucleic acid motif that functions as the protein translation initiation site in most eukaryotic mRNA transcripts. It ensures that a protein is correctly translated from the genetic message, mediating ribosome assembly and translation initiation.

What is the role of the eIF4F complex?

Here, we focus on the eIF4F complex, its role in chemoresistance, and its targeting with small-molecule inhibitors.

Are there any eIF4A inhibitors in the market?

Effector’s work on eIF4 to date has yielded zotatifin, an inhibitor of the eIF4A component of the complex that began phase 1/2 testing in patients with solid tumors late last year.

How does the eIF4F complex regulate mRNA translation?

The eIF4F complex regulates the cap-dependent mRNA translation process. It is becoming increasingly evident that aberrant activity of this complex is observed in many cancers, leading to the selective synthesis of proteins involved in tumor growth and metastasis.

Which is the most sensitive expression of eIF4A?

eIF4A is highly expressed in human primary leukemias Based on our previous work that identified AML cell lines as being most sensitive to eIF4A inhibition among various cancer types [ 33 ], we first surveyed public databases for evidence supporting the relevance of translation initiation in AML.