Which of the following groups can perform self-splicing?
Explanation: Self splicing is observed in two groups of very rare intron that are, group I and group II introns.
What is meant by self-splicing intron?
Self-splicing occurs for rare introns that form a ribozyme, performing the functions of the spliceosome by RNA alone. There are three kinds of self-splicing introns, Group I, Group II and Group III. Group I and II introns perform splicing similar to the spliceosome without requiring any protein.
How does self-splicing occur?
Self‑splicing occurs by a phosphoester transfer mechanism (Figure 3.3. 11) The 3’‑OH of the guanine nucleotide is the nucleophile that attacks and joins to the 5′ phosphate of the first nucleotide of the intron. There is no increase or decrease in the number of phosphoester bonds during this splicing.
What is the role of GTP in the self-splicing process?
Guanosine or guanine nucleotides serve as the attacking group in the self-splicing reactions of ribozymes.
What is splicing Class 12?
Splicing is the process of removal of introns and joining of exons in a definite manner.
What role do proteins play in the splicing of Group I self splicing introns in vivo?
What role do proteins play in the splicing of group I self-splicing introns in vivo? Proteins recruit spliceosome components to splice sites by binding to exonic-splicing enhancers. C. Together with snRNAs, proteins form the spliceosome complex.
How does self splicing occur?
How does RNA editing work?
RNA editing (also RNA modification) is a molecular process through which some cells can make discrete changes to specific nucleotide sequences within an RNA molecule after it has been generated by RNA polymerase. RNA editing occurs in the cell nucleus and cytosol, as well as within mitochondria and plastids.
What happens if introns are not removed?
During the process of splicing, introns are removed from the pre-mRNA by the spliceosome and exons are spliced back together. If the introns are not removed, the RNA would be translated into a nonfunctional protein. Splicing occurs in the nucleus before the RNA migrates to the cytoplasm.
What enzyme removes introns?
Spliceozymes: Ribozymes that Remove Introns from Pre-mRNAs in Trans.