What is the HindIII lambda phage size marker?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
Why is lambda DNA used as a marker?
Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
How many Ecori sites are there in lambda DNA?
six recognition sites
ABOUT THIS PRODUCT: The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease.
How many fragments will HindIII cut lambda DNA into?
The HindIII digest of lambda DNA yields at least 6 fragments suitable for use as molecular weight standards for gel electrophoresis. This is called a ladder.
What is Lambda HindIII ladder?
The λ HindIII DNA Ladder is prepared by restriction digestion of phage Lambda DNA to completion with HindIII, followed by heat inactivation of the enzyme. The resulting 7 fragments range from 564 base pairs to 23.13 kilobases in size. The λ Hind DNA Ladder is supplied ready to use in loading buffer containing dye.
What is lambda genome?
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. The head contains the phage’s double-strand linear DNA genome.
Does bacteriophage have DNA or RNA?
Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. Their genomes may encode as few as four genes (e.g. MS2) and as many as hundreds of genes.
How many nucleotides are in bacteriophage lambda?
The 12 nucleotide 5′ overhangs at the cos-ends of the linear Lambda DNA are the result of a cut by the enzyme terminase. This enzyme is encoded by Lambda itself and acts like a restriction enzyme during the replication of the phage DNA.
How many fragments are produced by EcoRI?
When cut by EcoRI, the cDNA will yield 0.5, 1.5, and 2 kilobase fragments. Digesting with both EcoRI and HindIII, will yield 0.5, 1, and 1.5 kilobase fragments.
How does bacteriophage lambda work?
Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot ‘force’ its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.
How is lambda DNA digested by EcoRI / HindIII?
Lambda DNA was completely digested by EcoRI/HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. The marker is ready-to-use.
When to use Thermo Scientific lambda DNA marker?
Thermo Scientific Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer.
How is lambda DNA used in agarose gels?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on…
How many base pairs are in the lambda DNA marker?
Lambda DNA was completely digested with EcoRI and HindIII, purified and dissolved in a storage buffer. The DNA marker contains the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125.