How is macrophage activation measured?
One of the most reliable ways to measure classical activation of murine macrophages is by their production of nitric oxide (NO). The assay for detecting NO, based on the Griess reagent, is simple and convenient, taking only ~30 min to complete.
What does macrophage activation mean?
In pediatric rheumatology, the term macrophage activation syndrome (MAS) refers to a condition caused by excessive activation and expansion of T lymphocytes and macrophagic histiocytes that exhibit hemophagocytic activity.
Are macrophages CD11b+?
Integrin αM (CD11b) is expressed in myeloid-lineage cells such as monocytes/macrophages, neutrophils, eosinophils, and basophils, and in lymphoid cells such as NK cells and peritoneal B-1 cells (Springer, 1994).
What are the activities of macrophages?
Macrophages are specialised cells involved in the detection, phagocytosis and destruction of bacteria and other harmful organisms. In addition, they can also present antigens to T cells and initiate inflammation by releasing molecules (known as cytokines) that activate other cells.
What happens in macrophage activation syndrome?
Macrophage activation syndrome is characterized by pancytopenia, liver insufficiency, coagulopathy, and neurologic symptoms and is thought to be caused by the activation and uncontrolled proliferation of T lymphocytes and well-differentiated macrophages, leading to widespread hemophagocytosis and cytokine …
What cells do macrophages activate?
Macrophages interact with T cells in order to bring about T cell activation in target organs, and are themselves activated by inflammatory messenger molecules (cytokines) produced by the T cells. Macrophages produce toxic chemicals, such as nitric oxide, that can kill surrounding cells.
What is Ly6G?
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow.
What is the function of CD11b?
CD11b modulates several key biological functions in innate immune cells, including cell adhesion, migration, and phagocytosis.
Can macrophages be activated by T cells?
Macrophages interact with T cells in order to bring about T cell activation in target organs, and are themselves activated by inflammatory messenger molecules (cytokines) produced by the T cells.