What other compounds could you test using the disk diffusion method?
In drug discovery laboratories, the disk diffusion test is used to screen natural product extracts for antibacterial activity. Extracts with antibacterial activity, for example the petroleum ether, chloroform, ethanol and acetone extracts above, will produce a zone of inhibition.
How do you prepare antimicrobial discs?
Prepare a solution with concentration 2 mg/ml. Then you put 50 microliters in the disc for 100 microgrammes, 25 microliter for 50 microgrammes.
How does disk diffusion assay work?
Disk diffusion by the Kirby-Bauer method is a standardized technique for testing rapidly growing pathogens. Briefly, a standardized inoculum (i.e., direct suspension of colonies to yield a standardized inoculum is acceptable) is swabbed onto the surface of MH agar (i.e., 150-mm plate diameter).
What is the name of the antibiotic disk diffusion test in this lab quizlet?
Natural antimicrobial agents produced by microorganisms. Also called the disk diffusion test, is a valuable standard tool for measuring the effectiveness of antimicrobics against pathogenic microorganisms. You just studied 62 terms!
How does the antibiotic get from the disk to the agar?
How does the antibiotic get from the disk into the agar? The antibiotic diffuses out of the disk and into the agar. This diffusion can be affected by temperature and depth of agar in the plate.
How do antibiotic discs dissolve?
- Use water.
- If it’s not heat sensitive, heat it up to 50 degrees and try again.
- Use half ethanol (95%) and try to dissolve, then slowly add half water.
- Finally, you could add 100% acetic acid and that should do it, however in a concentration of max 0.5%.
What would you expect to see if the chemicals used on a disk have no antimicrobial properties?
When the chemical on your disk has no effect, what would you expect to see on your plate? That bacteria will still grow. The bacteria will not and/or will partially grown on your plate.